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Issue 6, 2009
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Morphology-sensitive Raman modes of the malaria pigment hemozoin

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Resonance Raman spectroscopy was applied for investigating the malaria pigment hemozoin, which is an important target structure of antimalarialdrugs. Morphology-sensitive low wavenumber modes of hemozoin were selectively enhanced with help of excitation wavelengths at λ = 633 nm and λ = 647 nm. The assignment of the most prominent bands in the Raman spectra at 343 cm−1 and 368 cm−1 was assisted by DFT calculations of the hemozoin dimer. The mode at 343 cm−1 in the Raman spectrum of hemozoin is strongly enhanced with λexc. = 647 nm and is represented by a combined, symmetric doming mode of the two hematin units in the hemozoin dimer. The enhancement of this vibration is stronger in the resonance Raman spectrum of hemozoin compared with less crystalline β-hematin. The selective resonance enhancement of the morphology-sensitive Raman modes of hemozoin is caused by absorption bands in the UV-VIS-NIR spectrum. This absorption spectrum of the crystalline malaria pigment hemozoin shows a strong band at 655 nm. Another broad absorption band at 870 nm is the reason for the strong relative resonance enhancement of the mode at 1372 cm−1 in the Raman spectrum of crystalline hemozoin with λexc. = 830 nm. In conclusion, resonance Raman micro-spectroscopy with λexc. = 647 nm was shown to have great potential as an analytical tool to probe the morphology of hematin samples.

Graphical abstract: Morphology-sensitive Raman modes of the malaria pigment hemozoin

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Publication details

The article was received on 03 Dec 2008, accepted on 02 Apr 2009 and first published on 09 Apr 2009

Article type: Paper
DOI: 10.1039/B821705J
Citation: Analyst, 2009,134, 1126-1132
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    Morphology-sensitive Raman modes of the malaria pigment hemozoin

    T. Frosch, S. Koncarevic, K. Becker and J. Popp, Analyst, 2009, 134, 1126
    DOI: 10.1039/B821705J

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