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Issue 5, 2009
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Development of a voltammetric technique for monitoring brain dopamine metabolism: compensation for interference caused by DOPAC electrogenerated during homovanillic acid detection

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Abstract

The established stability of carbon-paste electrodes (CPEs) in brain extracellular fluid was exploited to develop a voltammetric technique to monitor the dopamine metabolite, homovanillic acid (HVA), at 10 s intervals. At the scan rates needed for this time resolution, 3,4-dihydroxyphenylacetic acid (DOPAC), electrogenerated as a result of HVA oxidation, was observed in the cyclic staircase voltammograms, and this interfered with the straightforward reliable quantification of HVA. However, correction of the HVA signal, recorded in mixtures, with currents from the DOPAC and ascorbate regions of the voltammogram allowed the reproducible construction of well behaved HVA calibration plots. These showed good linearity, LOD values, selectivity and stability during six days of continuous CPE exposure to a lipid medium, which served as an in-vitro model of CPE implantation in brain tissue for future applications.

Graphical abstract: Development of a voltammetric technique for monitoring brain dopamine metabolism: compensation for interference caused by DOPAC electrogenerated during homovanillic acid detection

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Article information


Submitted
17 Jun 2008
Accepted
18 Feb 2009
First published
05 Mar 2009

Analyst, 2009,134, 893-898
Article type
Paper

Development of a voltammetric technique for monitoring brain dopamine metabolism: compensation for interference caused by DOPAC electrogenerated during homovanillic acid detection

I. A. Mulla, J. P. Lowry, P. A. Serra and R. D. O'Neill, Analyst, 2009, 134, 893
DOI: 10.1039/B810227A

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