Issue 8, 2009

A simple and cost-effective method, as an appropriate alternative for visible spectrophotometry: development of a dopamine biosensor

Abstract

In this study, a new, simple, fast and inexpensive method as an alternative to visible spectrophotometry is developed. In this method the cells containing the sample solution were scanned with a scanner, then the color of each cell was analyzed with software written in visual basic (VB 6) media to red, green and blue values. The cells were built by creating holes in the Plexiglas® sheet. The dimensions of identical cells were examined by Cr (III) solution with known concentrations. The validity of this new method was studied by determination of dopamine (DA) without using any other reagent. The parameters which affect the system were optimized. The comparison between the current and traditional UV-Vis spectrophotometry methods was studied and the results revealed similar trends in both methods. The developed method was successfully applied to the determination of dopamine in serum and urine without using any pretreatment. Finally comparing the results obtained in the developed method showed that microwave irradiation of the solution can decrease the experimental time, increase sensitivity and improve the limit of detection.

Graphical abstract: A simple and cost-effective method, as an appropriate alternative for visible spectrophotometry: development of a dopamine biosensor

Article information

Article type
Paper
Submitted
21 Jan 2009
Accepted
20 May 2009
First published
09 Jun 2009

Analyst, 2009,134, 1692-1698

A simple and cost-effective method, as an appropriate alternative for visible spectrophotometry: development of a dopamine biosensor

A. Abbaspour, A. Khajehzadeh and A. Ghaffarinejad, Analyst, 2009, 134, 1692 DOI: 10.1039/B901273G

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