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Issue 1, 2009
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Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors

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Abstract

An extremely sensitive procedure for the isolation and detection of DNA from bacterial cell cultures is described. Direct asymmetric PCR amplified products from E. coli cultures are specifically detected, at a concentration level as low as 0.01 cfu mL−1 (cfu, colony forming unit), using disposable magnetic DNA hybridization amperometric sensors with no need for culture preconcentration steps.

Graphical abstract: Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors

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Publication details

The article was received on 02 Sep 2008, accepted on 23 Oct 2008 and first published on 04 Nov 2008


Article type: Communication
DOI: 10.1039/B815307H
Citation: Analyst, 2009,134, 34-37
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    Ultrasensitive detection of coliforms by means of direct asymmetric PCR combined with disposable magnetic amperometric genosensors

    Ó. A. Loaiza, S. Campuzano, M. Pedrero, P. García and J. M. Pingarrón, Analyst, 2009, 134, 34
    DOI: 10.1039/B815307H

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