Issue 1, 2009
From the journal:

Analyst

Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system

Hitoshi Shiku,*a   Shun Goto,a   Sungbong Jung,a   Kuniaki Nagamine,a   Masahiro Koide,b   Tomosato Itayama,b   Tomoyuki Yasukawac  and  Tomokazu Matsue*a  

* Corresponding authors

a Graduate School of Environmental Studies, Tohoku University, 6-6-11, Aramaki-Aoba, Sendai 980-8579, Japan
E-mail: shiku@bioinfo.che.tohoku.ac.jp, matsue@bioinfo.che.tohoku.ac.jp

b Environmental Chemistry Division, National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba 305-8506, Japan

c Graduate School of Material Science, University of Hyogo, Hyogo, Japan

Abstract

A highly sensitive and quantitative analysis was performed using a poly(dimethylsiloxane) (PDMS) microwell array in a scanning electrochemical microscopy setup. A microelectrode with a relatively large seal radius was used to cover the top of the cylindrical PDMS microwell (96 pL). The voltammogram for 4 mM ferrocyanide resulted in a charge value of 38 nC, suggesting that almost 100% of the reductant in the microwell was converted to the oxidation current. When genetically modified yeast cells were entrapped in the microwell, the accumulation of p-aminophenol (PAP) produced by expressing β-galactosidase (βGAL) was successfully observed.

Graphical abstract: Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system

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Article information

DOI
https://doi.org/10.1039/B808428A
Article type
Paper
Submitted
19 May 2008
Accepted
02 Sep 2008
First published
20 Oct 2008

Analyst, 2009,134, 182-187

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Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system

H. Shiku, S. Goto, S. Jung, K. Nagamine, M. Koide, T. Itayama, T. Yasukawa and T. Matsue, Analyst, 2009, 134, 182 DOI: 10.1039/B808428A

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