Issue 15, 2008
From the journal:

Organic & Biomolecular Chemistry

Enzymatic N-methylaminoacylation of tRNA using chemically misacylated AMP as a substrate†

Shinsuke Sando,*a   Hiroki Masu,a   Chika Furutania  and  Yasuhiro Aoyama*a  

* Corresponding authors

a Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, Kyoto, Japan
E-mail: ssando@sbchem.kyoto-u.ac.jp, aoyamay@sbchem.kyoto-u.ac.jp
Fax: +81-75-383-2767
Tel: +81-75-383-2766

Abstract

Using “chemically misacylated AMP” which is photogeneratable, tRNA can be enzymatically acylated with N-methylamino acids in an efficiency comparable to that of the corresponding natural aminoacylation process with amino acids.

Graphical abstract: Enzymatic N-methylaminoacylation of tRNA using chemically misacylated AMP as a substrate

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Article information

DOI
https://doi.org/10.1039/B806965D
Article type
Communication
Submitted
24 Apr 2008
Accepted
11 Jun 2008
First published
23 Jun 2008

Org. Biomol. Chem., 2008,6, 2666-2668

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Enzymatic N-methylaminoacylation of tRNA using chemically misacylated AMP as a substrate

S. Sando, H. Masu, C. Furutani and Y. Aoyama, Org. Biomol. Chem., 2008, 6, 2666 DOI: 10.1039/B806965D

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