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Issue 12, 2008
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An integrated microfluidic chip for chromosome enumeration using fluorescence in situ hybridization

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Abstract

Fluorescence in situ hybridization (FISH) is a powerful technique for probing the genetic content of individual cells at the chromosomal scale. Conventional FISH techniques provide a sensitive diagnostic tool for the detection of chromosomal alterations on a cell-by-cell basis; however, the cost-per-test in terms of reagent and highly qualified labour has prevented its wide-spread utilization in clinical settings. Here, we address the inefficient use of labour with the first integrated and automated on-chip FISH implementation, one that requires only minutes of setup time from the technician. Our microfluidic chip has lowered the reagent use by 20-fold, decreased the labour time by 10-fold, and substantially reduced the amount of support equipment needed. We believe this cost-effective platform will make sensitive FISH techniques more accessible for routine clinical usage.

Graphical abstract: An integrated microfluidic chip for chromosome enumeration using fluorescence in situ hybridization

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Publication details

The article was received on 21 Jul 2008, accepted on 17 Sep 2008 and first published on 23 Oct 2008


Article type: Technical Note
DOI: 10.1039/B812443D
Citation: Lab Chip, 2008,8, 2151-2156

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    An integrated microfluidic chip for chromosome enumeration using fluorescence in situ hybridization

    V. J. Sieben, C. S. Debes-Marun, L. M. Pilarski and C. J. Backhouse, Lab Chip, 2008, 8, 2151
    DOI: 10.1039/B812443D

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