Novel β-cyclodextrin modified organic polymeric monolithic substrate for solid phase extraction-room temperature phosphorescence

Abstract

An organic polymeric monolithic disk was prepared by situpolymerization of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA) in a binary porogenic solvent consisting 1-propanol and 1,4-butanediol, then modified by a reaction with β-cyclodextrin (β-CD). The β-CD modified disk can be used as a solid substrate and solid phase extraction (SPE) membrane for solid phase extraction-room temperature phosphorescence (SPE-RTP). The room temperature phosphorescence (RTP) behaviors of 14 organic compounds on the β-CD modified disk were examined. The results indicated that phenanthrene, 7,8-benzoquinoline, carbazole, fluorene, 3-indoleacetic acid and 1-naphthylacetic acid can emit strong RTP, and the limits of detection (LOD) were found to be 6.5 × 10⁻¹⁰–3.0 × 10⁻⁹ mol mL⁻¹. Used as solid phase extraction membrane, the modified disk could selectively enrich compounds with three rings such as phenanthrene, 7,8-benzoquinoline, carbazole and fluorene. After solid phase extraction, LODs of four compounds were decreased to 4.0 × 10⁻¹²–6.4 × 10⁻¹¹ mol mL⁻¹. The method was applied to the determination of fluorene and phenanthrene in biosamples with satisfactory results.