Issue 4, 2008
From the journal:

Analyst

Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays

M. T. Fernández-Argüelles,a   J. M. Costa-Fernández,a   R. Pereiroa  and  A. Sanz-Medel*a  

* Corresponding authors

a Department of Physical and Analytical Chemistry, University of Oviedo, Julián Clavería, 8, 33006 Oviedo, Spain
E-mail: asm@uniovi.es
Fax: +34 985 10 31 25
Tel: +34 985 10 34 74

Abstract

A simple procedure for the bio-conjugation of amphiphilic polymer-coated quantum dots with antibodies is described. The formed bio-conjugates are purified from an excess of free antibodies and of free nanoparticles by size-exclusion HPLC and then characterized by fluorescence emission and MALDI-TOFMS. The applicability of the approach is demonstrated for aflatoxin B1 detection.

Graphical abstract: Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays

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Article information

DOI
https://doi.org/10.1039/B802360N
Article type
Communication
Submitted
11 Feb 2008
Accepted
12 Feb 2008
First published
25 Feb 2008

Analyst, 2008,133, 444-447

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Simple bio-conjugation of polymer-coated quantum dots with antibodies for fluorescence-based immunoassays

M. T. Fernández-Argüelles, J. M. Costa-Fernández, R. Pereiro and A. Sanz-Medel, Analyst, 2008, 133, 444 DOI: 10.1039/B802360N

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