Issue 1, 2007

An optical sensor for reactive oxygen species: encapsulation of functionalised silica nanoparticles into silicate nanoprobes to reduce fluorophore leaching

Abstract

Sol–gel nanoprobes, also known as Photonic Explorer for Bioanalysis with Biologically Localised Embedding (PEBBLE), capable of performing in-vitrointracellular monitoring of reactive oxygen species have been developed using a modified form of 5(6)-carboxyfluorescein diacetate. A sol–gel matrix was selected for the design of the probes as it is photostable, optically transparent and chemically inert, and to minimise leaching of the dye from the porous matrix it was covalently immobilised to silica nanoparticles (15 nm). Using this approach, 0.1% of the dye was found to leach over a typical analysis time of 5 h and minimal photobleaching was observed. In addition, the nanoprobes were shown to respond to hydrogen peroxide, hydroxyl anions, nitric oxide, peroxynitrile and superoxide anions, obtaining limits of detection of 2.2, 1.1, 3.2, 1.1 and 1.1 nM respectively. The nanoprobes were subsequently introduced into bovine oviducts using a lipid transfection reagent (Escort IV™) and fluorescence was observed.

Graphical abstract: An optical sensor for reactive oxygen species: encapsulation of functionalised silica nanoparticles into silicate nanoprobes to reduce fluorophore leaching

Supplementary files

Article information

Article type
Paper
Submitted
06 Aug 2007
Accepted
24 Sep 2007
First published
02 Oct 2007

Analyst, 2007,133, 71-75

An optical sensor for reactive oxygen species: encapsulation of functionalised silica nanoparticles into silicate nanoprobes to reduce fluorophore leaching

V. J. Hammond, J. W. Aylott, G. M. Greenway, P. Watts, A. Webster and C. Wiles, Analyst, 2007, 133, 71 DOI: 10.1039/B711995J

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