Jump to main content
Jump to site search

Issue 1, 2007
Previous Article Next Article

ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starchextraction

Author affiliations

Abstract

The 2-(4′-isothiocyanate-2′-hydroxyphenyl)benzoxazole dye was successfully applied as label of rice proteins during the alkaline extraction of starch. Direct fluorescence measurements were used to observe the presence of proteins labelled in different steps of rice starch extraction. The results were compared to those obtained with the well-known biuret colorimetric test. Whereas the colorimetric test indicates the absence of protein after the third extraction step, the fluorescence emission of the conjugate could be observed in all extraction steps. The separation of different rice proteins could also be observed.

Graphical abstract: ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starch extraction

Back to tab navigation

Article information


Submitted
01 Aug 2006
Accepted
06 Oct 2006
First published
02 Nov 2006

Photochem. Photobiol. Sci., 2007,6, 99-102
Article type
Paper

ESIPT-exhibiting protein probes: a sensitive method for rice proteins detection during starch extraction

M. B. Cardoso, D. Samios, N. P. da Silveira, F. S. Rodembusch and V. Stefani, Photochem. Photobiol. Sci., 2007, 6, 99
DOI: 10.1039/B610999C

Social activity

Search articles by author

Spotlight

Advertisements