Issue 9, 2007
From the journal:

Organic & Biomolecular Chemistry

Synthesis and characterization of a new fluorescent probe for reactive oxygen species†

Belinda Heyne,a   Chad Beddiea  and  J. C. Scaiano*a  

* Corresponding authors

a University of Ottawa, Department of Chemistry, 10 Marie Curie, Ottawa, ON, Canada
E-mail: tito@photo.chem.uottawa.ca

Abstract

We report the development of a new fluorescence sensor for reactive oxygen species (ROS) based on a benzofurazan structure. The sensor, NBFhd, is initially non-fluorescent and reacts with peroxyl radicals by hydrogen transfer in an aqueous medium under neutral conditions to release the fluorescent N-methyl-4-amino-7-nitrobenzofurazan (NBF) and 1,4-benzoquinone. NBFhd shows excellent contrast and no interference in the region of cell autofluorescence and is a new tool to detect ROS in homogeneous and biological systems.

Graphical abstract: Synthesis and characterization of a new fluorescent probe for reactive oxygen species

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/B702618H
Article type
Paper
Submitted
19 Feb 2007
Accepted
12 Mar 2007
First published
02 Apr 2007

Org. Biomol. Chem., 2007,5, 1454-1458

Permissions

Request permissions

Synthesis and characterization of a new fluorescent probe for reactive oxygen species

B. Heyne, C. Beddie and J. C. Scaiano, Org. Biomol. Chem., 2007, 5, 1454 DOI: 10.1039/B702618H

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements