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Issue 12, 2007
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Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis

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Abstract

Two photon excited (TPE) fluorescence detection was applied to native fluorescence detection of aromatics in microchip electrophoresis (MCE). This technique was evaluated as an alternative to common one photon excitation in the deep UV spectral range. TPE enables fluorescence detection of unlabeled aromatic compounds, even in non-deep UV-transparent microfluidic chips. In this study, we demonstrate the proof of concept of native TPE fluorescence detection of small aromatics in commercial microfluidic glass chips. Label-free TPE fluorescence detection of native proteins and small aromatics in MCE was achieved within the micromolar concentration range, utilising 420 nm excitation light.

Graphical abstract: Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis

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Supplementary files

Article information


Submitted
17 Jul 2007
Accepted
19 Sep 2007
First published
10 Oct 2007

Lab Chip, 2007,7, 1841-1844
Article type
Technical Note

Two-photon excited fluorescence detection at 420 nm for label-free detection of small aromatics and proteins in microchip electrophoresis

P. Schulze, M. Schüttpelz, M. Sauer and D. Belder, Lab Chip, 2007, 7, 1841
DOI: 10.1039/B710762E

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