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Issue 12, 2007
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Polar stimulation and constrained cell migration in microfluidic channels

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Abstract

Asymmetrical delivery of stimuli to moving cells for perturbing spatially-heterogeneous intracellular signaling is an experimental challenge not adequately met by existing technologies. Here, we report a robust microfluidic platform allowing localized treatment of the front and/or back of moving cells which crawl through narrow channels that they completely occlude. The enabling technical element for this study is a novel design for precise, passive balancing of flow inside the microfluidic device by contacting two fluid streams before splitting them again. The microchannels constrain cell morphology and induce qualitative and quantitative changes in neutrophil chemotaxis that mimic cells crawling through tissues.

Graphical abstract: Polar stimulation and constrained cell migration in microfluidic channels

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Article information


Submitted
10 Jul 2007
Accepted
17 Aug 2007
First published
04 Sep 2007

Lab Chip, 2007,7, 1783-1790
Article type
Paper

Polar stimulation and constrained cell migration in microfluidic channels

D. Irimia, G. Charras, N. Agrawal, T. Mitchison and M. Toner, Lab Chip, 2007, 7, 1783
DOI: 10.1039/B710524J

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