Jump to main content
Jump to site search

Issue 9, 2007
Previous Article Next Article

Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites

Author affiliations

Abstract

Methods to probe the molecular structure of living cells are of paramount importance in understanding drug interactions and environmental influences in these complex dynamical systems. The coupling of an acoustic levitation device with a micro-Raman spectrometer provides a direct molecular probe of cellular chemistry in a containerless environment minimizing signal attenuation and eliminating the affects of adhesion to walls and interfaces. We show that the Raman acoustic levitation spectroscopic (RALS) approach can be used to monitor the heme dynamics of a levitated 5 µL suspension of red blood cells and to detect hemozoin in malaria infected cells. The spectra obtained have an excellent signal-to-noise ratio and demonstrate for the first time the utility of the technique as a diagnostic and monitoring tool for minute sample volumes of living animal cells.

Graphical abstract: Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites

Back to tab navigation

Publication details

The article was received on 09 May 2007, accepted on 10 Jul 2007 and first published on 03 Aug 2007


Article type: Paper
DOI: 10.1039/B706997A
Citation: Lab Chip, 2007,7, 1125-1131
  •   Request permissions

    Raman acoustic levitation spectroscopy of red blood cells and Plasmodium falciparum trophozoites

    L. Puskar, R. Tuckermann, T. Frosch, J. Popp, V. Ly, D. McNaughton and B. R. Wood, Lab Chip, 2007, 7, 1125
    DOI: 10.1039/B706997A

Search articles by author

Spotlight

Advertisements