Jump to main content
Jump to site search

Issue 11, 2007
Previous Article Next Article

Cell encapsulating droplet vitrification

Author affiliations


The capability to encapsulate single cells in droplets while retaining high cell viability (>90%) has great impact on tissue engineering, high-throughput screening, as well as clinical diagnostics and therapeutics. We demonstrate a novel method to vitrify a small number of cells using cell-encapsulating droplets. The method allows vitrification at low cryoprotectant concentration (1.5 M propanediol and 0.5 M trehalose), similar to that used in slow freezing protocols. The method was successfully applied to five different mammalian cell types: AML-12 hepatocytes, NIH-3T3 fibroblasts, HL-1 cardiomyocytes, mouse embryonic stem cells, and RAJI cells.

Graphical abstract: Cell encapsulating droplet vitrification

Back to tab navigation

Publication details

The article was received on 17 Apr 2007, accepted on 25 Jul 2007 and first published on 09 Aug 2007

Article type: Paper
DOI: 10.1039/B705809H
Citation: Lab Chip, 2007,7, 1428-1433

  •   Request permissions

    Cell encapsulating droplet vitrification

    U. Demirci and G. Montesano, Lab Chip, 2007, 7, 1428
    DOI: 10.1039/B705809H

Search articles by author