Issue 12, 2007

Integrated microfluidic cell culture and lysis on a chip

Abstract

We present an integrated microfluidic cell culture and lysis platform for automated cell analysis that improves on systems which require multiple reagents and manual procedures. Through the combination of previous technologies developed in our lab (namely, on-chip cell culture and electrochemical cell lysis) we have designed, fabricated, and characterized an integrated microfluidic platform capable of culturing HeLa, MCF-7, Jurkat, and CHO-K1 cells for up to five days and subsequently lysing the cells without the need to add lysing reagents. On-demand lysis was accomplished by local hydroxide ion generation within microfluidic chambers, releasing both proteinacious (GFP) and genetic (Hoescht-stained DNA) material. Sample proteins exposed to the electrochemical lysis conditions were immunodetectable (p53) and their enzymatic activity (HRP) was investigated.

Graphical abstract: Integrated microfluidic cell culture and lysis on a chip

Supplementary files

Article information

Article type
Paper
Submitted
06 Aug 2007
Accepted
27 Sep 2007
First published
19 Oct 2007

Lab Chip, 2007,7, 1689-1695

Integrated microfluidic cell culture and lysis on a chip

J. T. Nevill, R. Cooper, M. Dueck, D. N. Breslauer and L. P. Lee, Lab Chip, 2007, 7, 1689 DOI: 10.1039/B711874K

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