Quantitative on-chip determination of taurine in energy and sports drinks

Abstract

A new method for the quantitative determination of taurine in beverages by microchip electrophoresis was developed. A rapid and simple sample preparation procedure, only including two dilution steps and the addition of the fluorogenic labeling reagent NBD-Cl (4-chloro-7-nitrobenzofurazan), is applied. Using a home-built wavelength-resolved fluorescence detector, the separation and determination of the taurine derivative could be achieved in only 12 s, while the additional spectral information was utilized to ensure peak purity. Spanning from 0.1 to 50 mmol L⁻¹, the linear dynamic range of the applied method was adapted to the apparent contents in common taurine containing beverages. The smallest detectable amount of the taurine derivative actually injected into the separation channel was as low as 60 amol. The method was successfully validated by an independent liquid chromatographic method.