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Issue 43, 2007
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Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays

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Abstract

We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA–microgel conjugates can be explored for the development of DNA based bioassays and biosensors.

Graphical abstract: Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays

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Publication details

The article was received on 27 Jun 2007, accepted on 08 Aug 2007 and first published on 22 Aug 2007


Article type: Communication
DOI: 10.1039/B709817K
Chem. Commun., 2007, 4459-4461

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    Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA–microgel bioassays

    M. M. Ali, S. Su, C. D. M. Filipe, R. Pelton and Y. Li, Chem. Commun., 2007, 4459
    DOI: 10.1039/B709817K

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