Issue 4, 2007

Site-specific labeling of DNA–protein conjugates by means of expressed protein ligation

Abstract

Site-specific bioconjugation of protein thioesters with a DNA oligonucleotide was achieved by Expressed Protein Ligation (EPL) and the new thiol group formed upon EPL in the conjugate was selectively coupled with small molecule labels using maleimide chemistry.

Graphical abstract: Site-specific labeling of DNA–protein conjugates by means of expressed protein ligation

Supplementary files

Article information

Article type
Communication
Submitted
16 Oct 2006
Accepted
01 Dec 2006
First published
14 Dec 2006

Chem. Commun., 2007, 353-355

Site-specific labeling of DNA–protein conjugates by means of expressed protein ligation

M. Lovrinovic, L. Fruk, H. Schröder and C. M. Niemeyer, Chem. Commun., 2007, 353 DOI: 10.1039/B614978B

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