Issue 6, 2007
From the journal:

Analyst

Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

Tatsuya Munaka,a   Hirohisa Abe,a   Masaki Kanai,a   Takashi Sakamoto,b   Hiroaki Nakanishi,a   Shuichi Shoji,c   Shinya Kimura,d   Taira Maekawad  and  Akira Murakami*b  

* Corresponding authors

a Technology Research Laboratory, Shimadzu Corporation, 3-9-4, Hikari-dai, Seika-cho, Soraku-gun, Kyoto 619-0237, Japan

b Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan
E-mail: akiram@kit.ac.jp
Fax: +81-75-724-7814
Tel: +81-75-724-7814

c Major in Nanoscience and Nanoengneering, Waseda University, 3-4-1, Okubo, Shinjuku, Tokyo 169-8555, Japan

d Department of Transfusion Medicine and Cell Therapy, Kyoto University Hospital, 54Kawahara, Sakyo-ku, Kyoto 606-8507, Japan

Abstract

This paper describes the cultivation of primary cells in a microchamber and the real-time monitoring of small amounts of antibody secretion after the introduction of a minute amount of stimulus by a microinjector using time-resolved fluorescence anisotropy analysis.

Graphical abstract: Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

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Article information

DOI
https://doi.org/10.1039/B701049D
Article type
Communication
Submitted
23 Jan 2007
Accepted
22 Mar 2007
First published
02 Apr 2007

Analyst, 2007,132, 512-514

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Real-time monitoring of antibody secretion from B-cells on a microchip stimulated with a minute amount of mitogen

T. Munaka, H. Abe, M. Kanai, T. Sakamoto, H. Nakanishi, S. Shoji, S. Kimura, T. Maekawa and A. Murakami, Analyst, 2007, 132, 512 DOI: 10.1039/B701049D

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