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Issue 5, 2007
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Standardless identification of selenocystathionine and its γ-glutamyl derivatives in monkeypot nuts by 3D liquid chromatography with ICP-MS detection followed by nanoHPLC–Q-TOF-MS/MS

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Abstract

A three-step chromatographic procedure using orthogonal separation mechanisms (size-exclusion, cation-exchange and ion-pairing reversed phase) was developed to purify three low molecular weight selenospecies, including the major compound, from the aqueous extract of monkeypot (Lecythis minor) nuts. The following reversed-phase nanoHPLC–electrospray Q-TOF-MS/MS allowed the formal standardless identification of selenocystathionine and two isoforms of γ-glutamyl-selenocystathionine. This is the first MS and MS/MS-based formal evidence of the presence of these compounds in a biological sample.

Graphical abstract: Standardless identification of selenocystathionine and its γ-glutamyl derivatives in monkeypot nuts by 3D liquid chromatography with ICP-MS detection followed by nanoHPLC–Q-TOF-MS/MS

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Publication details

The article was received on 21 Dec 2006, accepted on 02 Mar 2007 and first published on 20 Mar 2007


Article type: Paper
DOI: 10.1039/B618637H
Analyst, 2007,132, 439-449

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    Standardless identification of selenocystathionine and its γ-glutamyl derivatives in monkeypot nuts by 3D liquid chromatography with ICP-MS detection followed by nanoHPLC–Q-TOF-MS/MS

    M. Dernovics, T. García-Barrera, K. Bierla, H. Preud'homme and R. Lobinski, Analyst, 2007, 132, 439
    DOI: 10.1039/B618637H

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