Issue 9, 2006
From the journal:

Organic & Biomolecular Chemistry

Sequence-selective osmium oxidation of DNA: efficient distinction between 5-methylcytosine and cytosine†

Akimitsu Okamoto,*a   Kazuki Tainakaa  and  Taku Kameia  

* Corresponding authors

a Department of Synthetic Chemistry and Biological Chemistry, Faculty of Engineering, Kyoto University, Kyoto, Japan
E-mail: okamoto@sbchem.kyoto-u.ac.jp
Fax: +81 75 383 2759
Tel: +81 75 383 2755

Abstract

5-Methylcytosine was distinguished from cytosine using the large difference of their osmium oxidation rates, and this reaction was applied to detection of the cytosine methylation status at a specific site of a long sequence using the formation of a bulge structure by hybridization with a guide DNA.

Graphical abstract: Sequence-selective osmium oxidation of DNA: efficient distinction between 5-methylcytosine and cytosine

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Article information

DOI
https://doi.org/10.1039/B600401F
Article type
Communication
Submitted
11 Jan 2006
Accepted
07 Mar 2006
First published
21 Mar 2006

Org. Biomol. Chem., 2006,4, 1638-1640

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Sequence-selective osmium oxidation of DNA: efficient distinction between 5-methylcytosine and cytosine

A. Okamoto, K. Tainaka and T. Kamei, Org. Biomol. Chem., 2006, 4, 1638 DOI: 10.1039/B600401F

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