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Issue 10, 2006
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A red-fluorescent substrate microarray for lipase fingerprinting

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Abstract

A lipase substrate microarray was obtained by printing aliphatic C2–C12 monoesters of (5R)- and (5S)-3-(5,6-dihydroxyhexyloxy)benzaldehyde by reductive alkylation on amine-functionalized glass slides coated with bovine serum albumin and a short PEG linker. The microarray features 12 substrates and their 66 possible binary mixtures spotted in a 9 × 36 spot array. Lipase reactions are detected by chemoselective NaIO4-oxidation of the 1,2-diol hydrolysis product to form an aldehyde, which is then tagged with the red-fluorescent dye rhodamine B sulfohydrazide 1. Specific fingerprints are produced by active enzymes. These experiments provide the first example of lipase fingerprinting using microarrays.

Graphical abstract: A red-fluorescent substrate microarray for lipase fingerprinting

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Supplementary files

Article information


Submitted
30 Jun 2006
Accepted
02 Aug 2006
First published
24 Aug 2006

Mol. BioSyst., 2006,2, 492-498
Article type
Paper

A red-fluorescent substrate microarray for lipase fingerprinting

J. Grognux and J. Reymond, Mol. BioSyst., 2006, 2, 492
DOI: 10.1039/B609275F

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