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Issue 10, 2006
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Development and characterization of an all-solid-state potentiometric biosensor array microfluidic device for multiple ion analysis

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Abstract

A microfluidic device with an all-solid-state potentiometric biosensor array was developed using microfabrication technology. The sensor array included a pH indicator, and potassium and calcium ion-selective microelectrodes. The pH indicator was an iridium oxide thin film modified platinum microelectrode and the iridium oxide was deposited by an electrochemical method. The potassium and calcium ion-selective microelectrodes were platinum coated with silicon rubber based ion-selective membranes with respectively potassium (valinomycin) and calcium (ETH 1001) ionophores. The detection system was integrated with a micro-pneumatic pump which can continuously drive fluids into the microchannel through sensors at flow rates ranging from 52.4 µl min−1 to 7.67 µl min−1. The sensor array microfluidic device showed near-Nernstian responses with slopes of 62.62 mV ± 2.5 mV pH−1, 53.76 mV ± 3 mV −log[K+]−1 and 25.77 mV ± 2 mV −log[Ca2+]−1 at 25 °C ± 5 °C, and a linear response within the pH range of 2–10, with potassium and calcium concentrations between 0.1 M and 10−6 M. In this study the device provided a convenient way to measure the concentration of hydrogen, potassium and calcium ions, which are important physiological parameters

Graphical abstract: Development and characterization of an all-solid-state potentiometric biosensor array microfluidic device for multiple ion analysis

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Publication details

The article was received on 06 Mar 2006, accepted on 06 Jul 2006 and first published on 14 Aug 2006


Article type: Paper
DOI: 10.1039/B603364D
Citation: Lab Chip, 2006,6, 1362-1368
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    Development and characterization of an all-solid-state potentiometric biosensor array microfluidic device for multiple ion analysis

    W. Liao, C. Weng, G. Lee and T. Chou, Lab Chip, 2006, 6, 1362
    DOI: 10.1039/B603364D

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