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Issue 6, 2006
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Timing controllable electrofusion device for aqueous droplet-based microreactors

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Abstract

This paper describes an electrofusion device for controlling the precise moment of fusion between droplets by applying an electric field. This device allows (i) accurate determination of the start of chemical/biological reactions, (ii) minimum contact of reactants with channel walls—eliminating surface absorption problems, (iii) easy fabrication and (iv) continuous observation of initiated reaction. We demonstrated the fusion of β-galactosidase and fluorescein di-β-D-galactopyranoside (FDG) droplets, and observed the enzymatic reaction using fluorescence microscopy. In addition, sequential fusion of pico-litre droplets was also accomplished.

Graphical abstract: Timing controllable electrofusion device for aqueous droplet-based microreactors

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Publication details

The article was received on 05 Dec 2005, accepted on 20 Mar 2006 and first published on 31 Mar 2006


Article type: Paper
DOI: 10.1039/B517178D
Citation: Lab Chip, 2006,6, 757-763

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    Timing controllable electrofusion device for aqueous droplet-based microreactors

    W. Tan and S. Takeuchi, Lab Chip, 2006, 6, 757
    DOI: 10.1039/B517178D

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