Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 21st October 2020 from 07:00 AM to 07:00 PM (BST).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.


Issue 10, 2006
Previous Article Next Article

A bio-barcode assay for on-chip attomolar-sensitivity protein detection

Author affiliations

Abstract

Functionalized nanoparticles hold great promise in realizing highly sensitive and selective biodetection. We report a single disposable chip which is capable of carrying out a multi-step process that employs nanoparticles—a bio-barcode assay (BCA) for single protein marker detection. To illustrate the capability of the system, we tested for the presence of prostate specific antigen (PSA) in buffer solution and goat serum. Detection was accomplished at PSA concentrations as low as 500 aM. This corresponds to only 300 copies of protein analytes using 1 µL total sample volume. We established that the on-chip BCA for PSA detection offers four orders of magnitude higher sensitivity compared to commercially available ELISA-based PSA tests.

Graphical abstract: A bio-barcode assay for on-chip attomolar-sensitivity protein detection

Back to tab navigation

Article information


Submitted
04 May 2006
Accepted
28 Jul 2006
First published
15 Aug 2006

Lab Chip, 2006,6, 1293-1299
Article type
Paper

A bio-barcode assay for on-chip attomolar-sensitivity protein detection

E. D. Goluch, J. Nam, D. G. Georganopoulou, T. N. Chiesl, K. A. Shaikh, K. S. Ryu, A. E. Barron, C. A. Mirkin and C. Liu, Lab Chip, 2006, 6, 1293
DOI: 10.1039/B606294F

Social activity

Search articles by author

Spotlight

Advertisements