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Issue 10, 2005
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Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium

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Abstract

This paper describes a platform for real-time sensing of dissolved oxygen in a flowing microfluidic environment using an oxygen-sensitive luminescent dye (platinum octaethylporphyrin ketone) integrated into a micro-oxygenator device. Using a phase-based detection method, the luminescent decay lifetime of the dye was consistent with the linear Stern–Volmer relationship using both gaseous and aqueous samples. Maximum sensor resolution varied between 120–780 ppb across a range of dissolved oxygen (DO) concentrations ranging from 0–42.5 ppm. The sensor was subsequently used to determine the convective mass-transfer characteristics of a multi-layer polydimethylsiloxane (PDMS) microfluidic oxygenator. The membrane-based oxygenator showed excellent agreement with an analytical convection model, and the integrated oxygen sensor was accurate across a wide range of tested flow rates (0.05–5 mL min−1). The device is unique for its ease of fabrication and highly flexible configuration, as well as the novel incorporation of oxygen delivery and detection in a single micro-device. Potential applications include tissue engineering, cell culturing, and miniaturized bio-assays that require the delivery and/or detection of precise quantities of oxygen within a microfluidic construct.

Graphical abstract: Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium

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Publication details

The article was received on 08 Jun 2005, accepted on 16 Aug 2005 and first published on 25 Aug 2005


Article type: Paper
DOI: 10.1039/B508097E
Citation: Lab Chip, 2005,5, 1059-1066
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    Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium

    A. P. Vollmer, R. F. Probstein, R. Gilbert and T. Thorsen, Lab Chip, 2005, 5, 1059
    DOI: 10.1039/B508097E

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