An integrated microfluidic device for influenza and other genetic analyses

R. Pal; M. Yang; R. Lin; B. N. Johnson; N. Srivastava; S. Z. Razzacki; K. J. Chomistek; D. C. Heldsinger; R. M. Haque; V. M. Ugaz; P. K. Thwar; Z. Chen; K. Alfano; M. B. Yim; M. Krishnan; A. O. Fuller; R. G. Larson; D. T. Burke; M. A. Burns

doi:10.1039/B505994A

An integrated microfluidic device for influenza and other genetic analyses

R. Pal,†^a M. Yang,†^a R. Lin,^a B. N. Johnson,^a N. Srivastava,^a S. Z. Razzacki,^a K. J. Chomistek,^a D. C. Heldsinger,^a R. M. Haque,^b V. M. Ugaz,^a P. K. Thwar,^a Z. Chen,^a K. Alfano,^a M. B. Yim,^a M. Krishnan,^a A. O. Fuller,^c R. G. Larson,^a D. T. Burke^d and M. A. Burns*^ae

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* Corresponding authors

^a Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA

^b Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA

^c Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI 48109, USA

^d Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA

^e Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI 48109, USA

Abstract

An integrated microfluidic device capable of performing a variety of genetic assays has been developed as a step towards building systems for widespread dissemination. The device integrates fluidic and thermal components such as heaters, temperature sensors, and addressable valves to control two nanoliter reactors in series followed by an electrophoretic separation. This combination of components is suitable for a variety of genetic analyses. As an example, we have successfully identified sequence-specific hemagglutinin A subtype for the A/LA/1/87 strain of influenza virus. The device uses a compact design and mass production technologies, making it an attractive platform for a variety of widely disseminated applications.

This article is part of the themed collection: Celebrating our 2020 Prize and Award winners

Lab on a Chip

An integrated microfluidic device for influenza and other genetic analyses

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