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Issue 10, 2005
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Integrated microsystem for dielectrophoretic cell concentration and genetic detection

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Abstract

We have directly integrated a continuous-flow, electrokinetic method of bacterial cell concentration with room temperature, sequence-specific genetic detection. The system we have developed traps cells from a continuous-flow sample stream via dielectrophoresis, providing a 160-fold increase in cell concentration. PDMS microvalves then define a 100 nL volume around the trapped cells to which cell lysis buffer and genetic detection components are introduced. Direct, optical detection of Escherichia coli MC1061 cells is then accomplished via the sequence-specific hybridization of an rRNA-directed optical molecular beacon. This integrated microsystem is capable of sequence-specific genetic detection of 25 cells within 30 min.

Graphical abstract: Integrated microsystem for dielectrophoretic cell concentration and genetic detection

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Article information


Submitted
27 Apr 2005
Accepted
16 Aug 2005
First published
31 Aug 2005

Lab Chip, 2005,5, 1053-1058
Article type
Paper

Integrated microsystem for dielectrophoretic cell concentration and genetic detection

E. T. Lagally, S. Lee and H. T. Soh, Lab Chip, 2005, 5, 1053
DOI: 10.1039/B505915A

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