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Issue 12, 2005
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On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel

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Abstract

A novel approach appropriate for rapid separation and immobilization of a single cell by concomitantly utilizing laser manipulation and locally thermosensitive hydrogelation is proposed in this paper. We employed a single laser beam as optical tweezers for separating a target cell and locating it adjacent to a fabricated, transparent micro heater. Simultaneously, the target cell is immobilized or partially entrapped by heating the thermosensitive hydrogel with the micro heater. The state of the thermosensitive hydrogel can be switched from sol to gel and gel to sol by controlling the temperature through heating and cooling by the micro heater. After other unwanted cells are removed by the high-speed cleaning flow in the microchannel, the entrapped cell is successfully isolated. It is possible to collect the immobilized target cell for analysis or culture by switching off the micro heater and releasing the cell from the entrapment. We demonstrated that the proposed approach is feasible for rapid manipulation, immobilization, cleaning, isolation and extraction of a single cell. The experimental results are shown here.

Graphical abstract: On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel

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Publication details

The article was received on 18 Feb 2005, accepted on 27 Sep 2005 and first published on 17 Oct 2005


Article type: Paper
DOI: 10.1039/B502546J
Citation: Lab Chip, 2005,5, 1399-1403
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    On chip single-cell separation and immobilization using optical tweezers and thermosensitive hydrogel

    F. Arai, C. Ng, H. Maruyama, A. Ichikawa, H. El-Shimy and T. Fukuda, Lab Chip, 2005, 5, 1399
    DOI: 10.1039/B502546J

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