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Issue 2, 2005
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Detection of phosphorus tagged carboxylic acids using HPLC-SF-ICP-MS

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Abstract

High performance liquid chromatography (HPLC) has been coupled with sector field inductively coupled plasma mass spectrometry (SF-ICP-MS) for the determination of maleic, sorbic and fumaric acids after derivatisation with the phosphorus containing reagent tris(2,4,6-trimethoxyphenyl)phosphonium propylamine (TMPP). This allowed 31P+ selective detection to be performed for these compounds, which are normally invisible to detection by ICP-MS at low concentrations. Optimal reagent conditions for the derivatisation of 0.1 mM maleic acid were: 1 mM TMPP; 10 mM 2-chloro-1-methylpyridinium iodide (CMPI); 11 mM triethylamine. The efficiency of the derivatisation reaction was estimated to be between 10–20% and detection limits, estimated as 3 times baseline noise, were 0.046 nmol for TMPP and 0.25 nmol for derivatised maleic acid, for a 5 μl injection.

Graphical abstract: Detection of phosphorus tagged carboxylic acids using HPLC-SF-ICP-MS

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Publication details

The article was received on 14 Oct 2004, accepted on 17 Dec 2004 and first published on 10 Jan 2005


Article type: Paper
DOI: 10.1039/B415962D
Citation: J. Anal. At. Spectrom., 2005,20, 75-80
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    Detection of phosphorus tagged carboxylic acids using HPLC-SF-ICP-MS

    A. J. Cartwright, P. Jones, J. Wolff and E. H. Evans, J. Anal. At. Spectrom., 2005, 20, 75
    DOI: 10.1039/B415962D

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