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Issue 7, 2005
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Near-real-time determination of hydrogen peroxide generated from cigarette smoke

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Abstract

The ability to monitor hydrogen peroxide (H2O2) in aqueous smoke extracts will advance our understanding of the relationship between cigarette smoke-induced oxidative stress, inflammation, and disease and help elucidate the pathways by which the various smoke constituents exert their pathogenic effects. We have demonstrated, for the first time, the measurement of H2O2 production from cigarette smoke without prior separation of the sample. Cigarettes were tested on a commercial smoking machine, such that the whole smoke or gas vapor phase was bubbled through phosphate buffered saline solution at pH 7.4. Aliquots of these solutions were analyzed using an Amplex Red/horseradish peroxidase fluorimetric assay that required only a 2 minute incubation time, facilitating the rapid, facile collection of data. Catalase was used to demonstrate the selectivity and specificity of the assay for H2O2 in the complex smoke matrix. We measured ∼7–8 μM H2O2 from two reference cigarettes (i.e., 1R4F and 2R4F). We also observed 9× more H2O2 from whole smoke bubbled samples compared to the gas vapor phase, indicating that the major constituent(s) responsible for H2O2 formation reside in the particulate phase of cigarette smoke. Aqueous solutions of hydroquinone and catechol, both of which are particulate phase constituents of cigarette smoke, generated no H2O2 even though they are free radical precursors involved in the production of reactive oxygen species in the smoke matrix.

Graphical abstract: Near-real-time determination of hydrogen peroxide generated from cigarette smoke

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Publication details

The article was received on 09 Feb 2005, accepted on 04 May 2005 and first published on 09 Jun 2005


Article type: Paper
DOI: 10.1039/B502061A
Citation: J. Environ. Monit., 2005,7, 681-687
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    Near-real-time determination of hydrogen peroxide generated from cigarette smoke

    F. Yan, S. Williams, G. D. Griffin, R. Jagannathan, S. E. Plunkett, K. H. Shafer and T. Vo-Dinh, J. Environ. Monit., 2005, 7, 681
    DOI: 10.1039/B502061A

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