Issue 37, 2005
From the journal:

Chemical Communications

Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism†

Tadashi Ema,*a   Toshiyuki Fujii,a   Misa Ozaki,a   Toshinobu Korenagaa  and  Takashi Sakai*a  

* Corresponding authors

a Department of Applied Chemistry, Faculty of Engineering, Okayama University, Tsushima, Okayama, Japan
E-mail: ema@cc.okayama-u.ac.jp
Fax: +81-86-251-8092
Tel: +81-86-251-8091

Abstract

The enantioselectivity of a Burkholderia cepacia lipase toward secondary alcohols could be both increased and decreased rationally by introducing only a single mutation on the basis of the mechanism proposed previously.

Graphical abstract: Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism

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Article information

DOI
https://doi.org/10.1039/B508244G
Article type
Communication
Submitted
10 Jun 2005
Accepted
22 Jul 2005
First published
12 Aug 2005

Chem. Commun., 2005, 4650-4651

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Rational control of enantioselectivity of lipase by site-directed mutagenesis based on the mechanism

T. Ema, T. Fujii, M. Ozaki, T. Korenaga and T. Sakai, Chem. Commun., 2005, 4650 DOI: 10.1039/B508244G

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