Biosynthesis of conjugatable saccharidic moieties of GM2 and GM3 gangliosides by engineered E. coli

Sébastien Fort; Lemonia Birikaki; Marie-Pierre Dubois; Tatiana Antoine; Eric Samain; Hugues Driguez

doi:10.1039/B500686D

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Biosynthesis of conjugatable saccharidic moieties of GM₂ and GM₃ gangliosides by engineered E. coli†

Sébastien Fort,*^a Lemonia Birikaki,^a Marie-Pierre Dubois,^a Tatiana Antoine,^a Eric Samain^a and Hugues Driguez^a

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* Corresponding authors

^a Centre de Recherches sur les Macromolécules Végétales, CERMAV-FR-CNRS 2607, affiliated to Université Joseph Fourier Grenoble, BP 53, 38041 Grenoble Cedex 9, France
E-mail: Sebastien.Fort@cermav.cnrs.fr
Fax: +33 (0)4 76 54 72 05
Tel: +33 (0)4 76 03 76 03

Abstract

Oligosaccharidic moieties of GM₂ and GM₃ gangliosides bearing an allyl or a propargyl aglycon, are efficiently biosynthesized on the gram scale by growing metabolically engineered Escherichia coli cells in the presence of the corresponding lactoside acceptors and sialic acid.

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Supplementary files

Experimental details and NMR spectra PDF (247K)

Article information

DOI: https://doi.org/10.1039/B500686D
Article type: Communication
Submitted: 17 Jan 2005
Accepted: 11 Mar 2005
First published: 22 Mar 2005

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Chem. Commun., 2005, 2558-2560

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Biosynthesis of conjugatable saccharidic moieties of GM₂ and GM₃ gangliosides by engineered E. coli

S. Fort, L. Birikaki, M. Dubois, T. Antoine, E. Samain and H. Driguez, Chem. Commun., 2005, 2558 DOI: 10.1039/B500686D

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