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Issue 2, 2005
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Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

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Abstract

One normally assumes that the analytical measurement process does not introduce spurious compounds. Capillary electrophoresis is a separation method frequently used for small-volume biological measurements. We demonstrate the potential for creating new peaks in a capillary electropherogram when using electrokinetic injections and illustrate the potential deleterious effects with biological samples involving serotonin and nitric oxide measurements. Specifically, when measuring the serotonin content from individual neurons using electrokinetic injections from 360 nL stainless steel vials, we detect a new peak that we identify as a serotonin dimer. We do not observe this peak when using hydrodynamic injections.

Graphical abstract: Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

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Publication details

The article was received on 24 Aug 2004, accepted on 10 Nov 2004 and first published on 07 Dec 2004


Article type: Paper
DOI: 10.1039/B413024C
Citation: Analyst, 2005,130, 147-151

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    Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

    J. N. Stuart, N. G. Hatcher, X. Zhang, R. Gillette and J. V. Sweedler, Analyst, 2005, 130, 147
    DOI: 10.1039/B413024C

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