Issue 2, 2005

Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

Abstract

One normally assumes that the analytical measurement process does not introduce spurious compounds. Capillary electrophoresis is a separation method frequently used for small-volume biological measurements. We demonstrate the potential for creating new peaks in a capillary electropherogram when using electrokinetic injections and illustrate the potential deleterious effects with biological samples involving serotonin and nitric oxide measurements. Specifically, when measuring the serotonin content from individual neurons using electrokinetic injections from 360 nL stainless steel vials, we detect a new peak that we identify as a serotonin dimer. We do not observe this peak when using hydrodynamic injections.

Graphical abstract: Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

Article information

Article type
Paper
Submitted
24 Aug 2004
Accepted
10 Nov 2004
First published
07 Dec 2004

Analyst, 2005,130, 147-151

Spurious serotonin dimer formation using electrokinetic injection in capillary electrophoresis from small volume biological samples

J. N. Stuart, N. G. Hatcher, X. Zhang, R. Gillette and J. V. Sweedler, Analyst, 2005, 130, 147 DOI: 10.1039/B413024C

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