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Issue 14, 2004
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Immunoassays for cortisol using antibody-doped sol–gel silica

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Abstract

Anti-cortisol was encapsulated in optically transparent sol–gel silica matrices and competitive immunoassays for cortisol were conducted using the antibody-doped silica material as sensing elements. Calibration curves were derived in the physiological relevant range for cortisol of 1 to 100 µg dl−1. Sensing elements were prepared in both monolith and thin-film forms, and results show that with thin films, accessibility of antigen to the encapsulated antibody was improved leading to a significant reduction in the required assay time. Moreover, non-specific binding to the sol–gel silica matrix was well controlled.

Graphical abstract: Immunoassays for cortisol using antibody-doped sol–gel silica

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Publication details

The article was received on 09 Feb 2004, accepted on 15 Apr 2004 and first published on 19 May 2004


Article type: Paper
DOI: 10.1039/B401902D
Citation: J. Mater. Chem., 2004,14, 2311-2316
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    Immunoassays for cortisol using antibody-doped sol–gel silica

    J. C. Zhou, M. H. Chuang, E. H. Lan, B. Dunn, P. L. Gillman and S. M. Smith, J. Mater. Chem., 2004, 14, 2311
    DOI: 10.1039/B401902D

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