Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance work on Wednesday 27th March 2019 from 11:00 AM to 1:00 PM (GMT).

During this time our website performance may be temporarily affected. We apologise for any inconvenience this might cause and thank you for your patience.

Issue 7, 2004
Previous Article Next Article

Investigation of the reaction of cisplatin with methionine in aqueous media using HPLC-ICP-DRCMS

Author affiliations


The reaction of cisplatin with methionine was studied by high performance ion chromatography coupled to inductively coupled plasma mass spectrometry equipped with dynamic reaction cell technique (HPLC-ICP-DRCMS) at two different cisplatin concentrations simulating chemotherapy conditions and waste water levels. The reaction of cisplatin with methionine was monitored over a period of 16 h. Accurate quantification of all platinum containing compounds was achieved via species unspecific on-line isotope dilution mass spectrometry. A limit of detection (LOD) of 0.31, 0.25, 3.83, 1.07, 0.56, 0.82 and 2.38 µg L−1 was calculated at m/z 194 for cisplatin, monoaquacisplatin, diaquacisplatin and the four platinum containing adducts, respectively. Stoichiometric platinum/sulfur ratios were assessed for characterization of the four adducts using ICP-DRCMS detection employing oxygen as reaction gas. An excellent sulfur detection limit of 1.30 µg L−1 could be achieved by HPLC-ICP-DRCMS (20 µL injection volume). At high cisplatin levels (0.6 mmol L−1) typical in chemotherapy, it was found that adducts show different kinetic behavior depending on the two investigated chloride levels (1.5 and 150 mmol L−1). Moreover, the reaction course depended on the concentration of the reactants, i.e. cisplatin and methionine. Experiments simulating possible reactions of the compounds in the aquatic environment revealed that at low µmol L−1 levels no adduct formation occurred. Finally, the stability of the four adducts potentially formed during chemotherapy was investigated representing the dilution of patient urine via hospital waste water. A considerable amount of highly active monoaquacisplatin was formed, indicating a reversal of detoxification reaction pathways of the human body.

Back to tab navigation

Publication details

The article was received on 10 Feb 2004, accepted on 15 Apr 2004 and first published on 15 Jun 2004

Article type: Paper
DOI: 10.1039/B402028F
Citation: J. Anal. At. Spectrom., 2004,19, 894-898

  •   Request permissions

    Investigation of the reaction of cisplatin with methionine in aqueous media using HPLC-ICP-DRCMS

    Zs. Stefánka, S. Hann, G. Koellensperger and G. Stingeder, J. Anal. At. Spectrom., 2004, 19, 894
    DOI: 10.1039/B402028F

Search articles by author