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Issue 7, 2004
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Retention characteristics of protonated mobile phases injected into deuterated mobile phases in capillary liquid chromatography (LC) using on-line nuclear magnetic resonance (NMR) detection

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Abstract

The behavior of protonated binary solvents injected into deuterated binary mobile phases in capillary LC is studied with NMR. Specifically, the solvent elution is followed on-flow with a capillary LC coupled to a 900 nL volume microcoil NMR probe. A range of identical composition 5% protonated (and 95% deuterated) solvents is injected into composition-matched deuterated mobile phases of CD3CN/D2O and CD3OD/D2O. The protonated components separate for all solvent combinations except at 80% CD3CN/20% D2O and ∼72% CD3OD/28% D2O where only a single retention time is observed. The more hydrophilic protonated component, HOD, elutes first with higher percentages of hydrophilic solvent, D2O, in the mobile phase whereas retention is reversed with the higher percentage of the more hydrophobic solvent (CD3CN and CD3OD) in the mobile phase. The hydrophilic/hydrophobic nature of the chromatographic system as a function of mobile phase composition is characterized by following the retention times of protonated solvents.

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Publication details

The article was received on 20 Nov 2003, accepted on 04 May 2004 and first published on 03 Jun 2004


Article type: Paper
DOI: 10.1039/B315029A
Citation: Analyst, 2004,129, 629-633

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    Retention characteristics of protonated mobile phases injected into deuterated mobile phases in capillary liquid chromatography (LC) using on-line nuclear magnetic resonance (NMR) detection

    D. A. Jayawickrama, A. M. Wolters and J. V. Sweedler, Analyst, 2004, 129, 629
    DOI: 10.1039/B315029A

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