Issue 12, 2003
From the journal:

Photochemical & Photobiological Sciences

Difference spectra measurement of squid rhodopsin in the submillimeter wave region

Akio Kishigami,*ab   Masatoshi Itoh,c   Tatsuo Nozokido,*cd   Hiroaki Minamide,c   Yasuo Tsukaharab  and  Koji Mizunocd  

* Corresponding authors

a Department of Physiology, School of Medicine, Nagoya University, Nagoya, Japan
E-mail: akio@med.nagoya-u.ac.jp
Fax: +81-52-7445015
Tel: +81-52-744-5016;

b Laboratory for Photo-biology, Photodynamics Research Center, The Institute of Physical and Chemical Research (RIKEN), Sendai, Japan

c Research Institute of Electrical Communication, Tohoku University, Sendai, Japan
E-mail: nozokido@riec.tohoku.ac.jp
Fax: +81-22-217-5516;
Tel: +81-22-217-5514

d Laboratory for Submillimeter-Wave Technology, Photodynamics Research Center, The Institute of Physical and Chemical Research (RIKEN), Sendai, Japan

Abstract

Theoretical studies by computer simulation have shown that vibrational modes, which depend on the subdomain motions of proteins, are located in the submillimeter wave region (i.e., 10–100 cm−1, 0.3–3 THz). We have successfully observed, by measuring the difference spectrum between squid rhodopsin and metarhodopsin to avoid water absorption, that squid rhodopsin shows its absorption features in this region. Our experimental results show that a native protein in solution indicates not only an absorbent property in this region but also an actual change of absorption with changes in protein conformation.

Graphical abstract: Difference spectra measurement of squid rhodopsin in the submillimeter wave region

  • This article is part of the themed collection: Vision
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Article information

DOI
https://doi.org/10.1039/B300197K
Article type
Paper
Submitted
07 Jan 2003
Accepted
16 Jan 2003
First published
05 Mar 2003

Photochem. Photobiol. Sci., 2003,2, 1303-1306

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Difference spectra measurement of squid rhodopsin in the submillimeter wave region

A. Kishigami, M. Itoh, T. Nozokido, H. Minamide, Y. Tsukahara and K. Mizuno, Photochem. Photobiol. Sci., 2003, 2, 1303 DOI: 10.1039/B300197K

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