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Issue 20, 2003
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The first fluorogenic assay for detecting a Baeyer–Villigerase activity in microbial cells

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Abstract

The first fluorogenic assay allowing for detection of microbial enzymes able to perform Baeyer–Villiger oxidation is described. This is based on the use of 4-oxopentyl umbelliferyl ether 1 as a fluorogenic substrate. When Baeyer–Villigerases active against this test ketone are present in the selected whole cells, 1 is transformed into 3-hydroxypropyl umbelliferyl ether 3, which, in a subsequent step, releases the fluorescent product umbelliferone. Different microorganisms, known to be endowed with Baeyer–Villigerase activity, were assayed.

Graphical abstract: The first fluorogenic assay for detecting a Baeyer–Villigerase activity in microbial cells

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Article information


Submitted
16 Jun 2003
Accepted
14 Jul 2003
First published
16 Sep 2003

Org. Biomol. Chem., 2003,1, 3500-3506
Article type
Paper

The first fluorogenic assay for detecting a Baeyer–Villigerase activity in microbial cells

M. C. Gutiérrez, A. Sleegers, H. D. Simpson, V. Alphand and R. Furstoss, Org. Biomol. Chem., 2003, 1, 3500
DOI: 10.1039/B306687H

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