Issue 20, 2003
From the journal:

Organic & Biomolecular Chemistry

The first fluorogenic assay for detecting a Baeyer–Villigerase activity in microbial cells

María C. Gutiérrez,a   Arthur Sleegers,a   Helen D. Simpson,a   Véronique Alphanda  and  Roland Furstoss*a  

* Corresponding authors

a Groupe Biocatalyse et Chimie Fine UMR-CNRS 6111, Université de la Méditerranée, Faculté des Sciences de Luminy, case 901, 163 Avenue de Luminy, 13288 Marseille, Cedex 9, France
E-mail: furstoss@luminy.univ-mrs.fr

Abstract

The first fluorogenic assay allowing for detection of microbial enzymes able to perform Baeyer–Villiger oxidation is described. This is based on the use of 4-oxopentyl umbelliferyl ether 1 as a fluorogenic substrate. When Baeyer–Villigerases active against this test ketone are present in the selected whole cells, 1 is transformed into 3-hydroxypropyl umbelliferyl ether 3, which, in a subsequent step, releases the fluorescent product umbelliferone. Different microorganisms, known to be endowed with Baeyer–Villigerase activity, were assayed.

Graphical abstract: The first fluorogenic assay for detecting a Baeyer–Villigerase activity in microbial cells

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Article information

DOI
https://doi.org/10.1039/B306687H
Article type
Paper
Submitted
16 Jun 2003
Accepted
14 Jul 2003
First published
16 Sep 2003

Org. Biomol. Chem., 2003,1, 3500-3506

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The first fluorogenic assay for detecting a Baeyer–Villigerase activity in microbial cells

M. C. Gutiérrez, A. Sleegers, H. D. Simpson, V. Alphand and R. Furstoss, Org. Biomol. Chem., 2003, 1, 3500 DOI: 10.1039/B306687H

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