Jump to main content
Jump to site search

Issue 3, 2003
Previous Article Next Article

Effects of liquid conductivity differences on multi-component sample injection, pumping and stacking in microfluidic chips

Author affiliations

Abstract

As an increasing number of processes are being integrated into Lab-on-a-chip devices, there is an increasing need for flexible and accurate sample manipulation techniques for effective transport and separation. Conductivity differences between running buffer and analyte samples can arise as a product of on-chip processing, or by design. The two situations studied here are sample pumping (where bulk transport is increased and separation of charged analytes is delayed using a relatively high conductivity sample), and sample stacking (where bulk transport is decreased and separation of charged analytes is expedited using a relatively low conductivity sample). A recently developed dynamic loading method for on-chip sample injection in a straight-cross channel configuration is applied here to both pumping and stacking cases. A key characteristic of the dynamic loading method is the ability to inject samples of high concentration density and uniformity of any length. By employing the conductivity differences alone, the effectiveness of either sample transport or sample separation are shown to improve over the uniform conductivity case. Then it is demonstrated that increasing the sample length, through dynamic loading, greatly increases the effectiveness of sample pumping, evidenced in an eight-fold increase in peak height as well as a decrease in total sample length at a downstream detector. Dynamic loading in the sample stacking case was shown to also increase peak intensity height (three-fold) in rapid separations. These results demonstrate that the dynamic loading technique, used in conjunction with strategic conductivity differences, significantly extends the capabilities of microfluidic chips.

Back to tab navigation

Publication details

The article was received on 25 Apr 2003, accepted on 20 Jun 2003 and first published on 07 Jul 2003


Article type: Paper
DOI: 10.1039/B304614A
Citation: Lab Chip, 2003,3, 173-179
  •   Request permissions

    Effects of liquid conductivity differences on multi-component sample injection, pumping and stacking in microfluidic chips

    D. Sinton, L. Ren, X. Xuan and D. Li, Lab Chip, 2003, 3, 173
    DOI: 10.1039/B304614A

Search articles by author

Spotlight

Advertisements