Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 3, 2003
Previous Article Next Article

Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay

Author affiliations

Abstract

A microfluidic device for counting and sizing particles and particle agglomerates based on laser light scattering is demonstrated. The particles were confined hydrodynamically and passed through a focused laser beam. Scattering at two different angles, 15° and 45°, was detected. At an acquisition rate of 10 kHz, a throughput of 150 particles s−1 was achieved. Scattering intensity was found to depend on particle volume for 2 to 9 µm diameter particles. Size discrimination of particles with a diameter ratio of 1 : 2 was accomplished. In addition, the scattering signals of particle agglomerates formed in a particle-enhanced immunoassay for C-reactive protein (CRP) were measured. Scattering intensity was found to be dependent on the CRP concentration, 100 ng CRP per mL could be detected. The particle counting method presented is generic and can be employed in a wide variety of assays as well as for cell counting and particle counting.

Graphical abstract: Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay

Back to tab navigation

Article information


Submitted
22 Jan 2003
Accepted
25 Apr 2003
First published
30 May 2003

Lab Chip, 2003,3, 187-192
Article type
Paper

Counting and sizing of particles and particle agglomerates in a microfluidic device using laser light scattering: application to a particle-enhanced immunoassay

N. Pamme, R. Koyama and A. Manz, Lab Chip, 2003, 3, 187
DOI: 10.1039/B300876B

Social activity

Search articles by author

Spotlight

Advertisements