Localized chemical stimulation to micropatterned cells using multiple laminar fluid flows

Abstract

Micropatterns of the electrically conjugating cardiomyocytes were prepared on a single cell level by microcontact printing (μCP), and the localized chemical stimulations were applied to the cellular pattern using multiple laminar flows. The locally delivered 1-octanol inactivated part of the myocyte patterns, while the other areas retained the activity showing spontaneous and synchronous pulsatility. Since both the cells and flows were well defined as micropatterns and each integrated on a chip, the obtained results simply demonstrate the cellular responses in a single-cell network.