Species-specific isotope dilution analysis (IDA) has been used to determine the mass fraction of methylmercury in a fish muscle certified reference material, DORM-2. Two spike solutions were prepared and their mass fractions determined by coupling high-performance liquid chromatography (HPLC) with both quadrupole (Q) and multicollector (MC) inductively coupled plasma mass spectrometry (ICP-MS) and uncertainty budgets calculated. For the Q-ICP-MS determination the relative standard uncertainty of the spike was 4.1%. The major uncertainty contribution (62%) arose from the uncertainties associated with the relative isotopic abundances of Hg isotopes as given by IUPAC. The measured isotope amount ratios (200Hg∶199Hg and 205Tl∶203Tl) contributed 37% of the combined uncertainty. For the multicollector instrument the relative standard uncertainty of the spike solution mass fraction was 0.7%. In this case the uncertainty budget was dominated by the Hg isotopic uncertainties, with a contribution of 99%. The uncertainty contributions from the measured isotope amount ratios were reduced to 0.7% of the total when using MC-ICP-MS. The two spike solutions were separately used to determine the mass fraction of methylmercury in DORM-2 CRM by species-specific IDA, using HPLC coupled with quadrupole ICP-MS. The values found were 4.45 ± 0.90 µg g−1 and 4.25 ± 0.47 µg g−1 using spikes characterised by Q- and MC-ICP-MS, respectively (the uncertainties quoted are the expanded uncertainty, k
= 2), compared with a certified value of 4.47 ± 0.32. The major uncertainty contribution for each determination was from the measured isotope amount ratios (70%) with lesser contributions from the uncertainties associated with the natural isotopic abundance of Hg and the spike mass fraction.
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