Issue 10, 2003
From the journal:

Physical Chemistry Chemical Physics

Ca2+ enhanced catalytic NADH oxidation: a coupled 31P-NMR and electrochemistry study

Nicolas Mano,†a   Alexander Kuhn,*a   Stéphane Menub  and  Erick J. Dufourcb  

* Corresponding authors

a Laboratoire d'Analyse Chimique par Reconnaissance Moléculaire, Ecole Nationale Supérieure de Chimie et de Physique de Bordeaux 16, Av Pey-Berland, 33607 Pessac, France
E-mail: kuhn@enscpb.fr
Fax: +33 5 40 00 27 17
Tel: +33 5 40 00 65 73

b Institut Européen de Chimie et Biologie, Ecole Polytechnique 16, Av Pey-Berland, 33607 Pessac, France

Abstract

The Ca2+-promoted 500% increase in activity of nitrofluorenone modified electrodes towards NADH oxidation is studied by 31P-NMR. The interaction of Ca2+ with NADH on the one hand and with the redox mediator on the other hand, results in a conformational change of the coenzyme. This event occurs concomitantly with the observed current increase. A three-member complex, in which Ca2+ bridges a NADH phosphate and the carboxyl group of the mediator is proposed as the molecular key for the potentiation of the electrocatalytic current.

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Article information

DOI
https://doi.org/10.1039/B212605B
Article type
Paper
Submitted
23 Dec 2002
Accepted
01 Apr 2003
First published
17 Apr 2003

Phys. Chem. Chem. Phys., 2003,5, 2082-2088

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Ca2+ enhanced catalytic NADH oxidation: a coupled 31P-NMR and electrochemistry study

N. Mano, A. Kuhn, S. Menu and E. J. Dufourc, Phys. Chem. Chem. Phys., 2003, 5, 2082 DOI: 10.1039/B212605B

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