Jump to main content
Jump to site search

Issue 1, 2003
Previous Article Next Article

Internal standard signal suppression by co-eluting analyte in isotope dilution LC-ESI-MS

Author affiliations

Abstract

The suppression of the internal standard by increasing concentrations of the co-eluting analyte in calibration series and plasma samples analysed by LC-ESI-MS was studied using the isotope dilution technique. A series of three analyte/deuterated analyte pairs including fexofenadine/d6-fexofenadine, dapsone/d4-dapsone and peudoephedrine/d3-ephedrine were investigated. Suppression of the internal standard signal was noticed in extracted plasma samples containing fexofenadine and d6-fexofenadine as internal standard, as well as in solvent based calibration solutions of the three pair of compounds noted above during LC-ESI-MS analysis at flow rates greater than 100 μL min−1. This signal suppression effect was described by invoking Enke’s model of electrospray ion generation. This model suggests that signal suppression can be ascribed to the competition between ionic species for charged surface sites present on the generated droplets during the electrospray process. The slopes of the calibration curves of the three analytes were close to unity (fexofenadien/d6-fexofenadine 0.964 ± 0.008, pseudoephedrine/d3-ephedrine 1.02 ± 0.080 and dapsone/d4-dapsone 0.905 ± 0.048) as predicted by the model, indicating that quantitation should not be affected by the variation in the peak area of the internal standard.

Back to tab navigation

Supplementary files

Article information


Submitted
27 Sep 2002
Accepted
08 Nov 2002
First published
25 Nov 2002

Analyst, 2003,128, 51-54
Article type
Paper

Internal standard signal suppression by co-eluting analyte in isotope dilution LC-ESI-MS

L. E. Sojo, G. Lum and P. Chee, Analyst, 2003, 128, 51
DOI: 10.1039/B209521C

Social activity

Search articles by author

Spotlight

Advertisements