Determination of some reactive oxygen species scavengers based on the peroxidase–oxidase oscillator

Abstract

This paper reports a new method for detection of ROS scavengers including superoxide dismutase, ascorbic acid and glutathione based on a ‘probe’ of peroxidase–oxidase biochemical oscillator. The oscillation period and amplitude change with different concentrations of scavengers. The linear ranges of superoxide dismutase, ascorbic acid and glutathione are respectively 1.56 × 10⁻⁴–1.56 × 10⁻³ mg mL⁻¹, 1.75 × 10⁻⁷–1.75 × 10⁻⁵ mol L⁻¹ and 9.38 × 10⁻⁷–7.5 × 10⁻⁵ mol L⁻¹. The selectivity, linearity and precision for superoxide dismutase, ascorbic acid, and glutathione are presented and discussed. The results compared well with other standard methods for determination of superoxide dismutase, ascorbic acid and glutathione. Some possible steps in the overall reaction mechanisms are discussed.