Jump to main content
Jump to site search

Issue 10, 2002
Previous Article Next Article

Rheology of glycocalix model at air/water interface

Author affiliations

Abstract

The rheology of glycocalix model compounds was studied using an interfacial stress rheometer (ISR) to understand the complex interplay of various forces (e.g. electrostatic interaction, van der Waals force, and hydrogen bonding) operating on cellular surfaces. The viscous and elastic surface moduli of the monolayer of synthetic glycolipids (named as Lac 1, 2, and 3) were measured as a function of the length of the linear oligosaccharide head groups quantitatively. The ISR device allows for highly sensitive and real-time measurements of viscoelastic parameters at different frequencies under controlled thermodynamic conditions (surface pressure, temperature). The Lac 1 monolayer was highly viscoelastic, which can be attributed to strong chain–chain correlations. The introduction of another lactose unit further reduced the chain–chain correlation, and so resulted in a fluid monolayer. In contrast, the Lac 3 monolayer exhibited a clear rheological transition from a viscous to an elastic film at the surface pressure of 6 ∼ 8 mN m−1. This rheological transition could be related to a thermodynamic phase transition to the liquid condensed phase, where the hydrating water is excluded and hydrogen bonding “bridges” the Lac 3 head groups through the film compression. This physical cross-linking of synthetic oligosaccharide chains observed here can model a generic function of glycocalix to stabilize the plasma membrane structure.

Back to tab navigation

Publication details

The article was received on 20 Nov 2001, accepted on 03 Jan 2002 and first published on 04 Apr 2002


Article type: Paper
DOI: 10.1039/B110631G
Phys. Chem. Chem. Phys., 2002,4, 1949-1952

  •   Request permissions

    Rheology of glycocalix model at air/water interface

    M. F. Schneider, K. Lim, G. G. Fuller and M. Tanaka, Phys. Chem. Chem. Phys., 2002, 4, 1949
    DOI: 10.1039/B110631G

Search articles by author

Spotlight

Advertisements